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rabbit polyclonal anti-adam 10  (Millipore)


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    Structured Review

    Millipore rabbit polyclonal anti-adam 10
    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
    Rabbit Polyclonal Anti Adam 10, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-adam+10/anti+adam10/pmc03030571-162-16-20
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-adam 10 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Central Angiotensin II Stimulation Promotes β Amyloid Production in Sprague Dawley Rats"

    Article Title: Central Angiotensin II Stimulation Promotes β Amyloid Production in Sprague Dawley Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0016037

    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
    Figure Legend Snippet: Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.

    Techniques Used: Expressing

    The same gels were blotted for β-actin as an internal standard. n = 6 for each group. ( A ) Representative enhanced chemiluminescence radiographs of immunoblots showing the immunoactivities of AT 1 R, APP, ADAM 10, BACE1, PS1, C83, C99 and β-actin. Density analysis showing levels of ( B ) AT 1 R, ( C ) APP, ( D ) ADAM 10, BACE1, and PS1, ( E ) ( F ) C83 and C99. Data in ( B–E ) were normalized by dividing the density of the AT 1 R, ADAM 10, BACE1, PS1, C83 and C99 bands by the density of the β-actin band. Data in ( F ) were normalized by dividing the density of the C83 and C99 band by that of the β-actin band and then the imAPP band. All data were represented as fold increases over the saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion. imAPP, immature APP; mAPP, mature APP; imADAM 10, immature ADAM 10;mADAM 10, mature ADAM 10.
    Figure Legend Snippet: The same gels were blotted for β-actin as an internal standard. n = 6 for each group. ( A ) Representative enhanced chemiluminescence radiographs of immunoblots showing the immunoactivities of AT 1 R, APP, ADAM 10, BACE1, PS1, C83, C99 and β-actin. Density analysis showing levels of ( B ) AT 1 R, ( C ) APP, ( D ) ADAM 10, BACE1, and PS1, ( E ) ( F ) C83 and C99. Data in ( B–E ) were normalized by dividing the density of the AT 1 R, ADAM 10, BACE1, PS1, C83 and C99 bands by the density of the β-actin band. Data in ( F ) were normalized by dividing the density of the C83 and C99 band by that of the β-actin band and then the imAPP band. All data were represented as fold increases over the saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion. imAPP, immature APP; mAPP, mature APP; imADAM 10, immature ADAM 10;mADAM 10, mature ADAM 10.

    Techniques Used: Western Blot

    Related Articles

    Western Blot:

    Article Title: Synaptic Autoregulation by Metalloproteases and ?-Secretase
    Article Snippet: Antibodies used were: mouse anti-PSD-95 antibody (1:500; clone K28.43 or clone K28/86.2; NeuroMab, Davis, CA), rabbit anti-GluA2/3 antibody (1 μg/ml; Millipore), mouse anti-δ-catenin (1/200; BD Biosciences), mouse anti-N-cadherin (1/100; BD Biosciences), mouse anti-synaptophysin (1/1000; Sigma-Aldrich), mouse anti-synaptotagmin 1 (1/100: Synaptic Systems), rabbit anti-MT5-MMP (cytoMT5-MMP, 1.5 μg/ml; #2850,) made in our lab and described previously ( Monea et al., 2006 ), rabbit Anti-EphB2 (1/1000 WB, Dr M. Greenberg), rabbit anti-ADAM 10 (1/200 for IF, 1/500 for WB, Sigma-Aldrich), Rabbit anti-ADAM 17 (1/200 for IF, 1/500 for WB, Abcam), mouse anti-BACE (1/100, 3D5, Dr Vassar), rabbit anti-presenilin N-terminal (1/500 IF and 1/1000 WB, PS1NT, Calbiochem), rabbit anti-presenilin loop domain (1/1000 WB, Calbiochem), rabbit anti-nicastrin (Dr. P. Fraser, 1/1000), anti-GluR1 (1/1000; Millipore), rabbit anti-tubulin (1/1500; Sigma-Aldrich), mouse anti-APP (clone C1/6.1 and m3.2, 1/1000, and for IF, 1/1000 Upstate).

    Article Title: Synaptic Autoregulation by Metalloproteases and -Secretase
    Article Snippet: Antibodies used were as follows: mouse antiPSD-95 antibody (1:500; clone K28.43 or clone K28/86.2; NeuroMab); rabbit anti-GluA2/3 antibody (1 g/ml; Millipore); mouse anti- - catenin (1/200; BD Biosciences); mouse anti-N-cadherin (1/100; BD Biosciences); mouse anti-synaptophysin (1/1000; Sigma-Aldrich); mouse anti-synaptotagmin 1 (1/100; Synaptic Systems); rabbit anti-MT5-MMP (1.5 g/ml; cytoMT5-MMP, catalog #2850) made in our laboratory and described previously (Monea et al., 2006); rabbit anti-EphB2 [1/1000 whole brain (WB), Dr. M. Greenberg, Harvard Medical School, Boston, MA]; rabbit anti-ADAM 10 [1/200 for immunofluorescence (IF), 1/500 for WB, Sigma-Aldrich]; rabbit anti-ADAM 17 (1/200 for IF, 1/500 for WB, Abcam); mouse anti-BACE (1/100, 3D5, Dr. R. Vassar, Northwestern University, Chicago, IL); rabbit anti-presenilin N-terminal (1/500 for IF and 1/1000 for WB, PS1NT, Calbiochem); rabbit anti-presenilin loop domain (1/1000 for WB, Calbiochem); rabbit anti-nicastrin (1/1000, Dr. P. Fraser, University of Toronto, Toronto, Canada); anti-GluR1 (1/1000; Millipore); rabbit anti-tubulin (1/1500; Sigma-Aldrich); mouse antiAPP (clone C1/6.1 and m3.2, 1/1000, and for IF, 1/1000, Millipore).

    Article Title: A diet containing grape powder ameliorates the cognitive decline in aged rats with a long-term high-fructose-high-fat dietary pattern.
    Article Snippet: Research has suggested that the consumption of foods rich in polyphenols is beneficial to the cognitive functions of the elderly.. We investigated the effects of grape consumption on spatial learning, memory performance, and neurodegeneration-related protein expression in aged rats fed a high-fructose-high-fat (HFHF) diet.. Six-week-old Wistar rats were fed an HFHF diet to 66 weeks of age to establish a model of an HFHF dietary pattern, before receiving intervention diets containing different amounts of grape powder for another 12 weeks in the second part of the experiment.

    Immunofluorescence:

    Article Title: Synaptic Autoregulation by Metalloproteases and ?-Secretase
    Article Snippet: Antibodies used were: mouse anti-PSD-95 antibody (1:500; clone K28.43 or clone K28/86.2; NeuroMab, Davis, CA), rabbit anti-GluA2/3 antibody (1 μg/ml; Millipore), mouse anti-δ-catenin (1/200; BD Biosciences), mouse anti-N-cadherin (1/100; BD Biosciences), mouse anti-synaptophysin (1/1000; Sigma-Aldrich), mouse anti-synaptotagmin 1 (1/100: Synaptic Systems), rabbit anti-MT5-MMP (cytoMT5-MMP, 1.5 μg/ml; #2850,) made in our lab and described previously ( Monea et al., 2006 ), rabbit Anti-EphB2 (1/1000 WB, Dr M. Greenberg), rabbit anti-ADAM 10 (1/200 for IF, 1/500 for WB, Sigma-Aldrich), Rabbit anti-ADAM 17 (1/200 for IF, 1/500 for WB, Abcam), mouse anti-BACE (1/100, 3D5, Dr Vassar), rabbit anti-presenilin N-terminal (1/500 IF and 1/1000 WB, PS1NT, Calbiochem), rabbit anti-presenilin loop domain (1/1000 WB, Calbiochem), rabbit anti-nicastrin (Dr. P. Fraser, 1/1000), anti-GluR1 (1/1000; Millipore), rabbit anti-tubulin (1/1500; Sigma-Aldrich), mouse anti-APP (clone C1/6.1 and m3.2, 1/1000, and for IF, 1/1000 Upstate).

    Article Title: Synaptic Autoregulation by Metalloproteases and -Secretase
    Article Snippet: Antibodies used were as follows: mouse antiPSD-95 antibody (1:500; clone K28.43 or clone K28/86.2; NeuroMab); rabbit anti-GluA2/3 antibody (1 g/ml; Millipore); mouse anti- - catenin (1/200; BD Biosciences); mouse anti-N-cadherin (1/100; BD Biosciences); mouse anti-synaptophysin (1/1000; Sigma-Aldrich); mouse anti-synaptotagmin 1 (1/100; Synaptic Systems); rabbit anti-MT5-MMP (1.5 g/ml; cytoMT5-MMP, catalog #2850) made in our laboratory and described previously (Monea et al., 2006); rabbit anti-EphB2 [1/1000 whole brain (WB), Dr. M. Greenberg, Harvard Medical School, Boston, MA]; rabbit anti-ADAM 10 [1/200 for immunofluorescence (IF), 1/500 for WB, Sigma-Aldrich]; rabbit anti-ADAM 17 (1/200 for IF, 1/500 for WB, Abcam); mouse anti-BACE (1/100, 3D5, Dr. R. Vassar, Northwestern University, Chicago, IL); rabbit anti-presenilin N-terminal (1/500 for IF and 1/1000 for WB, PS1NT, Calbiochem); rabbit anti-presenilin loop domain (1/1000 for WB, Calbiochem); rabbit anti-nicastrin (1/1000, Dr. P. Fraser, University of Toronto, Toronto, Canada); anti-GluR1 (1/1000; Millipore); rabbit anti-tubulin (1/1500; Sigma-Aldrich); mouse antiAPP (clone C1/6.1 and m3.2, 1/1000, and for IF, 1/1000, Millipore).

    Article Title: A diet containing grape powder ameliorates the cognitive decline in aged rats with a long-term high-fructose-high-fat dietary pattern.
    Article Snippet: Research has suggested that the consumption of foods rich in polyphenols is beneficial to the cognitive functions of the elderly.. We investigated the effects of grape consumption on spatial learning, memory performance, and neurodegeneration-related protein expression in aged rats fed a high-fructose-high-fat (HFHF) diet.. Six-week-old Wistar rats were fed an HFHF diet to 66 weeks of age to establish a model of an HFHF dietary pattern, before receiving intervention diets containing different amounts of grape powder for another 12 weeks in the second part of the experiment.



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    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
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    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
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    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
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    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
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    Millipore rabbit polyclonal anti-adam 10 (ab19026)
    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.
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    Image Search Results


    Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.

    Journal: PLoS ONE

    Article Title: Central Angiotensin II Stimulation Promotes β Amyloid Production in Sprague Dawley Rats

    doi: 10.1371/journal.pone.0016037

    Figure Lengend Snippet: Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.

    Article Snippet: The following primary antibodies and working dilutions were used: rabbit polyclonal anti-APP carboxyl terminal (Sigma, 1∶5,000), rabbit polyclonal anti-ADAM 10 (Millipore, 1∶500), rabbit monoclonal anti-BACE1 (Cell Signaling, 1∶500), mouse monoclonal anti-PS1 that recognizes the PS1 amino terminal (Abcam, 1∶250), mouse monoclonal anti-AT 1 R, which recognizes the AT 1 R carboxyl terminal (Abcam, 1∶125), and mouse monoclonal anti-β actin (Sigma, 1∶1,000).

    Techniques: Expressing

    The same gels were blotted for β-actin as an internal standard. n = 6 for each group. ( A ) Representative enhanced chemiluminescence radiographs of immunoblots showing the immunoactivities of AT 1 R, APP, ADAM 10, BACE1, PS1, C83, C99 and β-actin. Density analysis showing levels of ( B ) AT 1 R, ( C ) APP, ( D ) ADAM 10, BACE1, and PS1, ( E ) ( F ) C83 and C99. Data in ( B–E ) were normalized by dividing the density of the AT 1 R, ADAM 10, BACE1, PS1, C83 and C99 bands by the density of the β-actin band. Data in ( F ) were normalized by dividing the density of the C83 and C99 band by that of the β-actin band and then the imAPP band. All data were represented as fold increases over the saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion. imAPP, immature APP; mAPP, mature APP; imADAM 10, immature ADAM 10;mADAM 10, mature ADAM 10.

    Journal: PLoS ONE

    Article Title: Central Angiotensin II Stimulation Promotes β Amyloid Production in Sprague Dawley Rats

    doi: 10.1371/journal.pone.0016037

    Figure Lengend Snippet: The same gels were blotted for β-actin as an internal standard. n = 6 for each group. ( A ) Representative enhanced chemiluminescence radiographs of immunoblots showing the immunoactivities of AT 1 R, APP, ADAM 10, BACE1, PS1, C83, C99 and β-actin. Density analysis showing levels of ( B ) AT 1 R, ( C ) APP, ( D ) ADAM 10, BACE1, and PS1, ( E ) ( F ) C83 and C99. Data in ( B–E ) were normalized by dividing the density of the AT 1 R, ADAM 10, BACE1, PS1, C83 and C99 bands by the density of the β-actin band. Data in ( F ) were normalized by dividing the density of the C83 and C99 band by that of the β-actin band and then the imAPP band. All data were represented as fold increases over the saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion. imAPP, immature APP; mAPP, mature APP; imADAM 10, immature ADAM 10;mADAM 10, mature ADAM 10.

    Article Snippet: The following primary antibodies and working dilutions were used: rabbit polyclonal anti-APP carboxyl terminal (Sigma, 1∶5,000), rabbit polyclonal anti-ADAM 10 (Millipore, 1∶500), rabbit monoclonal anti-BACE1 (Cell Signaling, 1∶500), mouse monoclonal anti-PS1 that recognizes the PS1 amino terminal (Abcam, 1∶250), mouse monoclonal anti-AT 1 R, which recognizes the AT 1 R carboxyl terminal (Abcam, 1∶125), and mouse monoclonal anti-β actin (Sigma, 1∶1,000).

    Techniques: Western Blot